• Corinna Singleman

    Doctoral Candidate at CUNY Graduate Center

  •  

    “Somewhere, something incredible is waiting to be known.”

    Carl Sagan

     

    I have always sought to understand the world around me and am compelled to share what I’ve learned with anyone who will listen. My goal is to explore the world, gather what knowledge I can and spread that knowledge as far as it can go. Science and research provide the best paths to attaining my goals. 

     

    Currently, I am a doctoral candidate working on my thesis studying the effects of toxins found in NYC water ways on fish development. This project will determine the consequences of polychlorinated biphenyl (PCB) exposure on zebrafish and sturgeon heart development, specifically exploring a novel mechanism of aryl hydrocarbon receptor (AhR) action in causing these defects. My study marries the widely different fields of developmental, genetic and ecological biology.

    In the future I plan to use my expertise and scientific training to bridge the ever-widening gap between the scientific community and the rest of the world by teaching, writing and working on projects that directly impact my community.

  • EXPERIENCE

     

    CUNY Graduate Center

    Doctoral Candidate 

    Aug 2011 – Present

     

    I am a doctoral student studying the developmental consequences of PCBs on zebrafish and sturgeon heart development.

    Queens College CUNY

    Graduate Teaching Assistant 

    Jun 2012 – Present

    Adjunct Lecturer 

    Aug 2009 – Aug 2011

      

    Currently teach multiple lab sections for Anatomy and Physiology. Taught laboratory sections for Intro Bio, A&P and Nutritional Microbiology. Coauthored, with course head, new musculoskeletal lab for Intro Biology. Responsibilities include modifying and presenting lab lectures, writing and administering exams and maintaining regular office hours.

     

    Queens College

    Lab Technician 

    Aug 2009 – Aug 2011

     

    Conducted independent research on cardiomyopathy in zebrafish. Managed many lab support functions, including tending zebrafish test subjects, general upkeep of fish facility and laboratory training of other researchers in the laboratory.

    Chatham University

    Teaching Assistant for Cadaver Lab 

    Jan 2009 – May 2009

     

    Taught a weekly lab class identifying anatomy of male and female cadavers.

    Chatham University

    Supplemental Instructor for Intro to Cell Biology Course 

    Jan 2009 – May 2009

     

    Held weekly office hours to assist students. Sessions included lecture and reading material review, and included exposing students to study and understanding techniques to aid them in self-study.

    US Army Corps of Engineers

    Plover Steward 

    Jul 2008 – Sep 2008

     

    Worked in a team locating and monitoring piping plover and least tern nesting sites on private property on Long Island NY. Helped erect symbolic fencing and predator exclosures at nesting areas, monitoring reproductive success throughout the breeding season, recorded specified daily field observations including nesting success reports and time reports and assisted with preparation of final reports.

  • Peer-Reviewed Articles

    Growth and Maturation in the Zebrafish, Danio rerio: A Staging Tool for Teaching and Research.

    Corinna Singleman and Nathalia Holtzman

    July 2014

     

    Zebrafish have been increasingly used as a teaching tool to enhance the learning of many biological concepts from genetics, development and behavior to the understanding of the local watershed. While traditionally, in both research and teaching, zebrafish work has focused on embryonic stages; however later stages, from larval through adulthood, are increasingly being examined. Defining developmental stages based on age is a problematic way to assess maturity because many environmental factors, such as temperature, population density and water quality, impact growth and maturation. Fish length and characterization of key external morphological traits are considered better markers for maturation state. While a number of staging series exist for zebrafish, here we present a simplified normalization table of post-embryonic maturation well suited to both educational and research use. Specifically, we utilize fish size and four easily identified external morphological traits (pigment pattern, tail fin, anal fin and dorsal fin morphology) to describe three larval stages, a juvenile stage, and an adult stage. These simplified maturation standards will be a useful tool for both educational and research protocols.

     

    http://online.liebertpub.com/doi/abs/10.1089/zeb.2014.0976

    Analysis of Post-Embryonic Heart Development and Maturation in the Zebrafish, Danio rerio.

    Corinna Singleman and Nathalia Holtzman

    Nov 2012

     

    Background: Cardiac maturation is vital for animal survival and must occur throughout the animal's life. Zebrafish are increasingly used to model cardiac disease; however, little is known about how the cardiovascular system matures. We conducted a systematic analysis of cardiac maturation from larvae through to adulthood and assessed cardiac features influenced by genetic and environmental factors. Results: We identified a novel step in cardiac maturation, termed cardiac rotation, where the larval heart rotates into its final orientation within the thoracic cavity with the atrium placed behind the ventricle. This rotation is followed by linear ventricle growth and an increase in the angle between bulbous arteriosus and the ventricle. The ventricle transitions from a rectangle, to a triangle and ultimately a sphere that is significantly enveloped by the atrium. In addition, trabeculae are similarly patterned in the zebrafish and humans, both with muscular fingerlike projections and muscle bands that span the cardiac chamber. Of interest, partial loss of atrial contraction in myosin heavy chain 6 (myh6/weahu423/+) mutants result in the adult maintaining a larval cardiac form. Conclusions: These findings serve as a foundation for the study of defects in cardiovascular development from both genetic and environmental factors.

     

    http://onlinelibrary.wiley.com/doi/10.1002/dvdy.23882/abstract

    Heart dissection in larval, juvenile and adult zebrafish, Danio rerio.

    Corinna Singleman and Nathalia Holtzman

    Sept 2011

     

    In order to analyze heart development over the fish's lifespan, we dissect zebrafish hearts at numerous stages and photograph them for further analysis. This protocol explains two distinct, size dependent dissection techniques for zebrafish, ranging from larvae 3.5 mm standard length (SL) with hearts of 100 μm ventricle length (VL), to adults, with SL of 30 mm and VL 1mm or larger.Larval and adult fish have quite distinct body and organ morphology. Larvae are not only significantly smaller, they have less pigment and each organ is visually very difficult to identify. For this reason, we use distinct dissection techniques. We used pre-dissection fixation procedures, as we discovered that hearts dissected directly after euthanization have a more variable morphology, with very loose and balloon like atria compared with hearts removed following fixation. This protocol will provide a valuable technique for the study of cardiac development maturation and aging.

     

    http://www.jove.com/video/3165/heart-dissection-in-larval-juvenile-and-adult-zebrafish-danio-rerio?id=3165

  • EDUCATION

     

    CUNY Graduate Center

    Doctor of Philosophy (PhD) Biology, EEB (Ecology, Evolution & Behavior) 2011 - 2017

     

    Activities:

    DSC Representative for Biology

    Member of Biology EEB Advisory Committee & Executive Committee

    SETAC CDC member

     

    Memberships:

    Society for Ecotoxicology and Chemistry

    Genetics Society of America,

    New York Academy of Science

    Chatham College

    Master of Science (MS) Biology, General 2008 - 2009

     

     

    Macaulay Honors College at The City University of New York

    Bachelor of Arts (B.A.) Biology, General 2004 - 2008

     

    Macaulay Honors College Scholar at Queens College

    Activities:

    Biology & Chemistry tutor

    Biology Honors Society President

    Golden Key member

    Fencing Team Captain

  • MANTRA

    "If we knew what we were doing, it would not be called research, would it?"

    Albert Einstein

  • Honors & Awards

    Bain Graduate Fellowship

    2015-2016

    The Hudson River Foundation's Bain Graduate Fellowship supports graduate student research for a full academic year. My proposal focused on studying the underlying mechanism driving PCB derived heart defects in Atlantic sturgeon and zebrafish.

    Doctoral Student Research Grant #10

    2015

    This grant supported by the CUNY Graduate Center provides students with travel funds. I plan to use these funds to attend two meetings in the spring of 2016. 

    Tibor T. Polgar Fellowship

    2014

    The Hudson River Foundation's Polgar Fellowship is a summer grant awarded to students conducting research related to the Hudson River in NY. My proposal included exploring the consequences of PCB exposure to larval sturgeon hearts at the organ and cellular levels.

    Doctoral Student Research Grant #8

    2013

    This grant supported by the CUNY Graduate Center provides students with travel funds. I utilized this award to travel the the 11th International Conference on Zebrafish Development in June 2014 where I presented my work on zebrafish. 

    Seymour Fogel Endowment Fund Research Award

    2012

    The Fogel Endowment Fund supports PhD student genetic research using genetic and/or genomic tools. I used these funds to purchase supplies to study the consequences of PCB exposure on zebrafish development. 

    Summer Program for Undergraduate Research

    Summer 2006 & 2007

    The Howard Hughes Medical Institute supports undergraduate research during the summer months with a stipend for students conducting research full time. 

  • Service & Volunteer work

    Reviewer on Editorial board of BECT

    2016 - Present

    Member of Career Development Committee and Buddy Mentorship Subcommittee

    2015 - Present

    Doctoral Student Council - Biology Student Rep

    2015-2016

    Alumna Mentor for current MHC students

    2012 - 2015

    Judge for the NY State Science and Engineering Fair

    2013, 2014, 2015 & 2016

    Session I Moderator for Mid-Atlantic Region Zebrafish Meeting 

    May 2013

    Volunteer educator presenting scientific information to primary school aged children

    Oct 2012

    Volunteer human cadaver dissector for male and female cadavers. 

    2008 - 2009

    Dispatcher for College Point Volunteer Ambulance Corps

    2004 - 2008

  • SKILLS

     

    Developmental Biology Research

    Ecotoxicology Research

    Writing & Editing

  • CONTACT ME! 

    You can also find me at www.linkedin.com/in/CorinnaSingleman